Cloning, Expression and Assay
In last century achievements of recombinant DNA technology has revolutionized modern biology and accomplish significant dynamic growth. This century target based drug design also expected to achieve the same. Our laboratory is fully equipped to clone express purify and establish assay for validated targets for screening NCEs.
In last century achievements of recombinant DNA technology has revolutionized modern biology and accomplish significant dynamic growth. This century target based drug design also expected to achieve the same. Our laboratory is fully equipped to clone express purify and establish assay for validated targets for screening NCEs.
Research Areas
Target Discovery to screening
The shift in focus from ligand based design approaches to target based discovery over the last two to three decades has been a major milestone in drug discovery research. Currently, it is witnessing another major paradigm shift by leaning towards the holistic systems based approaches rather the reductionist single molecule based methods. The effect of this new trend is likely to be felt strongly in terms of new strategies for therapeutic intervention, new targets individually and in combinations, and design of specific and safer drugs. Computational modeling and simulation form important constituents of new-age biology because they are essential to comprehend the large-scale data generated by high-throughput experiments and to generate hypotheses, which are typically iterated with experimental validation.
The shift in focus from ligand based design approaches to target based discovery over the last two to three decades has been a major milestone in drug discovery research. Currently, it is witnessing another major paradigm shift by leaning towards the holistic systems based approaches rather the reductionist single molecule based methods. The effect of this new trend is likely to be felt strongly in terms of new strategies for therapeutic intervention, new targets individually and in combinations, and design of specific and safer drugs. Computational modeling and simulation form important constituents of new-age biology because they are essential to comprehend the large-scale data generated by high-throughput experiments and to generate hypotheses, which are typically iterated with experimental validation.
Genomics
The phenomenon ‘caloric restriction’ (CR; a diet in which calories are limited by 30-40% compared with animals fed ad libitum ) alters normal physiology in mammals and imparts many other health benefits. However, therapeutic application of CR in humans lacks feasibility. We are exploiting the usefulness of model organism (S. cerevisise and Rat) and effectiveness of microarray to identify promising CR mimetic candidates, thus saving time and cost for initial screening.
The phenomenon ‘caloric restriction’ (CR; a diet in which calories are limited by 30-40% compared with animals fed ad libitum ) alters normal physiology in mammals and imparts many other health benefits. However, therapeutic application of CR in humans lacks feasibility. We are exploiting the usefulness of model organism (S. cerevisise and Rat) and effectiveness of microarray to identify promising CR mimetic candidates, thus saving time and cost for initial screening.
Proteomics
Calorie restriction (CR) decreases oxidative stress in a variety of species and has been the focus of much interest lately. However the mechanisms of CR remain to be clearly elucidated. Using proteomics techniques we are studying oxidative stress regulation in yeast and rat models.
Calorie restriction (CR) decreases oxidative stress in a variety of species and has been the focus of much interest lately. However the mechanisms of CR remain to be clearly elucidated. Using proteomics techniques we are studying oxidative stress regulation in yeast and rat models.
Systems Biology
Identification and comparison of signaling and regulatory pathways that regulate cells' response to environmental stimuli. We integrate gene expression, genome sequence and epigenomic data from multiple species to discover essential regulatory pathways underlying fundamental biological processes such as oxidative stress and Calorie restriction.
Identification and comparison of signaling and regulatory pathways that regulate cells' response to environmental stimuli. We integrate gene expression, genome sequence and epigenomic data from multiple species to discover essential regulatory pathways underlying fundamental biological processes such as oxidative stress and Calorie restriction.
Modeling and in silico screening
We are also equipped to perform Protein modeling and virtual screening on targets using quantum chemical and thermodynamic based approaches. Computer aided drug design and in-silico screening are also employed in the rational design of small molecules. Screening of compound library were done on regular basis for identification of small molecule agonist/antagonist.
We are also equipped to perform Protein modeling and virtual screening on targets using quantum chemical and thermodynamic based approaches. Computer aided drug design and in-silico screening are also employed in the rational design of small molecules. Screening of compound library were done on regular basis for identification of small molecule agonist/antagonist.
